Mercuric chloride affects morphology and thymidine incorporation into DNA in astroglial cells in culture
By Ronnback, L. - Hansson, E.
| Record number: | 1665139 |
|---|---|
| Category: | animal use alternatives - brain - mercuric chloride |
Primary astroglial cell cultures from neonatal rat cerebral cortex revealed morphological changes when incubated in HgCl2 at various concentrations. After incubation for 30 minutes with 5 X 10(-6) HgCl2, most cells changed their form from a flat to a more rounded shape and extended processes. After longer incubations (>60 minutes) or at higher concentrations (greater than or equal to 10(-5)M), vacuoles appeared in many cells.[3H]-Thymidine incorporation into DNA was slightly increased after acute incubation in 10(-9)M HgCl2,while decreases were obtained after incubation in the micromolar range. The results are important due to the functions of astroglia during development and in the mature nervous system.
Issued: Apr 1991
Journal Title: Alternatives to laboratory animals
ISSN: 0261-1929
Volume issue: 19 v. 2 no.
Pages: 219 - 221
Notes:
Paper presented at the "Eighth Congress on In Vitro Toxicology of the Scandinavian Society for Cell Toxicology," Sept. 13-16, 1990, Norway.
AGRICOLA identifier: IND20355266
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