Magnetic resonance imaging assessment of a murine model of recessive polycystic kidney disease

By Sun, Y. - Zhou, J. - Stayner, G. - Munasinghe, J. - Shen, X. - Beier, D.R. - Albert, M.S.

Purpose: The pathogenesis of polycystic kidney disease (PKD) has not been firmly established; however, our current knowledge of cystogenesis and human cystic disease has been greatly advanced by a variety of animal models of PKD. To study the onset and degree of cyst formation in PKD mouse models without requiring sacrifice of these animals, we have initiated magnetic resonance imaging (MRI) studies of the juvenile cystic kidney (jck) mouse model. Methods: The MRI experiments were performed by use of a Bruker 8.5 T system, on seven-week-old mice that were homozygous for the recessive jck mutation and that manifested PKD. Kidney volume was measured, using three-dimensional segmentation postprocessing techniques. Results: The MR images of the enlarged kidneys from affected mice had regions of high signal intensity, with a radial distribution, which reflected the dilated collecting ducts observed in the corresponding histologic slices. The volume of PKD-affected kidney was about 4 times greater than that of the normal kidney. Conclusions: Magnetic resonance imaging has the ability to non-invasively assess cystic disease in mouse models of PKD. Of considerable importance is the opportunity to characterize this disease without sacrificing the animal. The three-dimensional MRI segmentation method provides accurate calculation of renal volume.