Use of the SOS-chromotest spot assay as a screening system for detecting genotoxic compounds in crude plant extracts
By Arrieta Baez, D. - Zepeda Vallejo, G. - Cano Sanchez, P. - Brena Valle, M. - Reyes Chilpa, R. - Jinenez Estrada, M.
| Record number: | 2928513 |
|---|---|
| Category: | Fabaceae - plant extracts - screening - gene expression - rapid methods - genotoxicity - bioflavonoids - quercetin - rutoside - antineoplastic agents - animal use alternatives - animal use replacement |
An SOS-chromotest spot assay was used to detect genotoxic compounds in crude plant extracts. The method allows simultaneous testing of extracts from different species in either a liquid or a solid crystalline form. Extracts from two species of the genus Senna, native to the state of Morelos, Mexico, were assayed. Four genotoxic compounds were isolated, and were identified as quercetin and rutin from S. wislizeni, and 5,7-di-O-methylrutin and 5,7-di-O-methylquercetin from S. skinneri. The SOS-chromotest spot assay proved to be useful for activity-guided fractionation at the beginning of screening for genotoxic compounds in crude plant extracts.
Issued: Jan/Feb 2002
Journal Title: Alternatives to laboratory animals
ISSN: 0261-1929
Volume issue: 30 v. 1 no.
Pages: 87 - 92
AGRICOLA identifier: IND23279751
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