Three-dimensional growth of renal epithelial cells in vitro: a tool in toxicity testing
By Andersen, K.J. - Christensen, E.I. - Vik, H.
| Record number: | 1667865 |
|---|---|
| Category: | animal use alternatives - kidneys - cell culture |
The tissue culture of multicellular spheroids from the renal epithelial cell line LLC-PK1 (proximal tubule) is described. This represents a biological system of intermediate complexity between renal tissue in vivo and simple monolayer cultures. The multicellular structures, which show many similarities to kidney tubules in vivo, including a vectorial water transport, should prove useful for studying the potential nephrotoxicity of drugs and chemicals in vitro. In addition, the propagation of renal epithelial cells as multicellular spheroids in serum-free culture may provide information on the release of specific biological parameters, which may be suppressed or masked in serum-supplemented media.
Issued: Apr 1993
Journal Title: Alternatives to laboratory animals
ISSN: 0261-1929
Volume issue: 21 v. 2 no.
Pages: 191 - 195
Notes:
Paper presented at the tenth annual Scandinavian Society of Cell Toxicology conference on in vitro toxicology, Sept 10-13, 1992, Visby, Gotland, Sweden.
AGRICOLA identifier: IND20359611
Thanks for your feedback! Please note that we cannot reply to you unless you send us an email.
What are you looking for?
We value your feedback so we can improve the information on the page. Please add your email address if you would like a reply. Thank you in advance for your help.!
Please contact us by email if you have any questions.