Three-dimensional growth of renal epithelial cells in vitro: a tool in toxicity testing
By Andersen, K.J. - Christensen, E.I. - Vik, H.
| Record number: | 1667865 |
|---|---|
| Category: | animal use alternatives - kidneys - cell culture |
The tissue culture of multicellular spheroids from the renal epithelial cell line LLC-PK1 (proximal tubule) is described. This represents a biological system of intermediate complexity between renal tissue in vivo and simple monolayer cultures. The multicellular structures, which show many similarities to kidney tubules in vivo, including a vectorial water transport, should prove useful for studying the potential nephrotoxicity of drugs and chemicals in vitro. In addition, the propagation of renal epithelial cells as multicellular spheroids in serum-free culture may provide information on the release of specific biological parameters, which may be suppressed or masked in serum-supplemented media.
Issued: Apr 1993
Journal Title: Alternatives to laboratory animals
ISSN: 0261-1929
Volume issue: 21 v. 2 no.
Pages: 191 - 195
Notes:
Paper presented at the tenth annual Scandinavian Society of Cell Toxicology conference on in vitro toxicology, Sept 10-13, 1992, Visby, Gotland, Sweden.
AGRICOLA identifier: IND20359611
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