Chemical tests as alternatives to animal tests in research on late symptoms in diabetes
By Nielsen, M. - Clausen, J.
| Record number: | 2928550 |
|---|---|
| Category: | diabetes - animal use alternatives - chemical reactions - animal models - antioxidants - ascorbic acid - vitamin E - glucose - biochemical pathways - free radicals - animal use replacement |
Glycation reactions, such as those seen in late diabetes, can be mimicked in purely chemical systems. The glycation is time-dependent, and in in vitro systems it may continue for days. Ascorbate seems to enhance the reactions. The reactions are associated with free-radical formation through transformation of an Amadori product to (deoxy-)glycoson, catalysed by heavy metals. Ascorbate enhances the reaction by a factor of 5-10 compared with in vitro systems without ascorbate. In vitro systems containing bovine serum albumin retard the formation of free-radicals, because of the formation of advanced glycation products.
Issued: May/June 2002
Journal Title: Alternatives to laboratory animals
ISSN: 0261-1929
Volume issue: 30 v. 3 no.
Pages: 341 - 348
Notes:
Paper presented at the 19th annual workshop of the Scandinavian Society for Cell Toxicology, September 6-9, 2001, Ringsted, Denmark.
AGRICOLA identifier: IND23336553
Thanks for your feedback! Please note that we cannot reply to you unless you send us an email.
What are you looking for?
We value your feedback so we can improve the information on the page. Please add your email address if you would like a reply. Thank you in advance for your help.!
Please contact us by email if you have any questions.