Chemical tests as alternatives to animal tests in research on late symptoms in diabetes
By Nielsen, M. - Clausen, J.
| Record number: | 2928550 |
|---|---|
| Category: | diabetes - animal use alternatives - chemical reactions - animal models - antioxidants - ascorbic acid - vitamin E - glucose - biochemical pathways - free radicals - animal use replacement |
Glycation reactions, such as those seen in late diabetes, can be mimicked in purely chemical systems. The glycation is time-dependent, and in in vitro systems it may continue for days. Ascorbate seems to enhance the reactions. The reactions are associated with free-radical formation through transformation of an Amadori product to (deoxy-)glycoson, catalysed by heavy metals. Ascorbate enhances the reaction by a factor of 5-10 compared with in vitro systems without ascorbate. In vitro systems containing bovine serum albumin retard the formation of free-radicals, because of the formation of advanced glycation products.
Issued: May/June 2002
Journal Title: Alternatives to laboratory animals
ISSN: 0261-1929
Volume issue: 30 v. 3 no.
Pages: 341 - 348
Notes:
Paper presented at the 19th annual workshop of the Scandinavian Society for Cell Toxicology, September 6-9, 2001, Ringsted, Denmark.
AGRICOLA identifier: IND23336553
Takk for din tilbakemelding! Vær oppmerksom på at vi ikke kan kontakte deg hvis ikke du oppgir din epostadresse.
Hva lette du etter?
Gi oss gjerne en tilbakemelding slik at vi kan forbedre informasjonen på siden. På forhånd takk for hjelpen! Vennligst skriv inn din epostadresse hvis du vil ha et svar.
Kontakt oss gjerne på e-post hvis du har spørsmål.